Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Reusing/Recycling Probes?

    Hi all,

    Just wondering if anyone has had any luck with successfully, or trying to, recycle/reuse DNA probes? Any advice, ideas, or insights would help.

    Thanks!

  • #2
    Why would you want to do that? This (to a non-bench scientist) sounds like a bad idea.

    Comment


    • #3
      I figure to save money on probes, but also, if this is something that can be done (relatively) "easily" and efficiently, it could allow for using recycled probes as a control variable for assay development?

      Comment


      • #4
        Do you mean biotinylated sequence capture probes?

        Comment


        • #5
          Originally posted by GenoMax View Post
          Why would you want to do that? This (to a non-bench scientist) sounds like a bad idea.
          This (to a bench scientist) also sounds like a bad idea. In theory, you can strip the sequence capture probes. In practice, you'll get contaminating sequences from previous captures.

          Comment


          • #6
            Compared to the money you saved, you will get more trouble in terms of library contamination and possible bias of probe contents.

            Comment


            • #7
              What kind of probes are you asking about? I re-use DIG-labeled PCR product probes for Southern blots.

              Comment

              Latest Articles

              Collapse

              • seqadmin
                Strategies for Sequencing Challenging Samples
                by seqadmin


                Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
                03-22-2024, 06:39 AM
              • seqadmin
                Techniques and Challenges in Conservation Genomics
                by seqadmin



                The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

                Avian Conservation
                Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
                03-08-2024, 10:41 AM

              ad_right_rmr

              Collapse

              News

              Collapse

              Topics Statistics Last Post
              Started by seqadmin, Yesterday, 06:37 PM
              0 responses
              10 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, Yesterday, 06:07 PM
              0 responses
              9 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, 03-22-2024, 10:03 AM
              0 responses
              49 views
              0 likes
              Last Post seqadmin  
              Started by seqadmin, 03-21-2024, 07:32 AM
              0 responses
              67 views
              0 likes
              Last Post seqadmin  
              Working...
              X