Hi all,
My aim is to do targeted sequencing.
I wish to use the TruSeq DNA library prep kit for multiplexing and then use the Roche-Nilmblegen sequence capture method.
As Illumina has changed the protocol by adding the indexes to the adapters, I guess the oligos used in the Roche protocol (Hybridisation enhancement oligos etc) need to be modified.
The problem I do not have access to the Illumina TruSeq sequences namely; adapter-index oligos and the primer sequences.
Can some one share these sequences?
Thanks
KiranKM
My aim is to do targeted sequencing.
I wish to use the TruSeq DNA library prep kit for multiplexing and then use the Roche-Nilmblegen sequence capture method.
As Illumina has changed the protocol by adding the indexes to the adapters, I guess the oligos used in the Roche protocol (Hybridisation enhancement oligos etc) need to be modified.
The problem I do not have access to the Illumina TruSeq sequences namely; adapter-index oligos and the primer sequences.
Can some one share these sequences?
Thanks
KiranKM
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