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Old 10-27-2020, 11:06 AM   #21
Pab77
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Location: Seattle

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Hi Rosmano, These look amazing! I think all of these should give you good resolution. Did you use 50K cells, protease inhibitor, and double-sided size selection? I've been working on C. elegans ATAC-seq with a titration of nuclei but not getting the nice laddering. For C. elegans, several papers used ~1-2 million nuclei for ATAC-seq library prep. However, not working in our hands. I'm using Buenstrostro protocol. Any input would be greatly appreciated.
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