SEQanswers

Go Back   SEQanswers > Bioinformatics > Bioinformatics



Similar Threads
Thread Thread Starter Forum Replies Last Post
Pac Bio fastq file quality score encoding lankage Pacific Biosciences 7 06-19-2015 06:26 AM
average quality score for fastq file jgibbons1 Bioinformatics 12 07-16-2013 01:13 PM
PacBio RS quality score output definition (FASTQ) vamiyn Pacific Biosciences 3 07-16-2013 02:44 AM
Detecting FASTQ Quality Score Format bhan Bioinformatics 2 01-02-2012 03:40 PM
Fastq quliaty score and MAQ output quality score baohua100 Bioinformatics 1 02-19-2009 09:21 AM

Reply
 
Thread Tools
Old 07-29-2019, 02:09 PM   #1
Jayson H
Junior Member
 
Location: Salt Lake City

Join Date: Aug 2011
Posts: 1
Default FASTQ changing quality score to zero for primer sequences

I want to mask primer sequences from analysis, but I want to keep the sequences in the FASTQ to help with alignment. Searching against a primer list and changing the quality scores to zero for matching bases would effectively prevent those bases from being included in analysis, and the preserved bases would aid in aligning the target sequence. Is there a method or tool to do this?
Jayson H is offline   Reply With Quote
Reply

Tags
fastq quality

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 01:31 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO