Tweet
Hi, at the risk of exposing my ignorance, a question: can someone point me to a reference that established the use of 30X coverage as a goal in sequencing? Where does this number come from? Why not 10X or 50X?
-
-
Usually related to the papers that used SNP arrays and NGS and tested the level of sequencing needed to call all heterozygote SNPs detected on the array by NGS or sanger sequencing. The first good example I remember is the Ventor diploid genome paper in Plos One but a number of similar examples are available. Scan the genome papers that also have a SNP array in the materials and methods and check their supplemental tables. But most I remember show diploid concordance >95% is not reached till around 26x or greater coverage
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Comment