Hi all,
We are examining cost cutting measures and considering moving to NEBNext Quick DNA Library prep. We have a 454 Junior and all of our kits and reagents are for rapid libraries. With NEBNext I have to order the adaptors separately. When I go to the IDT website, the only rapid library option for oligos includes the MIDs, which is fine. When I click on one of them, I tells me there is an Adaptor A and B. However, in the Roche rapid library protocol and the NEBNext protocol, when they refer to the adaptor ligation, it sounds like there is a single adaptor - not A and B. In the rapid library kits, there is a single tube of adaptor. The NEBNext protocol at the adaptor ligation step says to "add 1.0ul of adaptor to the reaction tube" with no mention of concentration. Am I supposed to mix MID adaptors we purchased from IDT in equimolar concentrations then add 1ul of that? At what concentration?
Thanks for any enlightenment.
We are examining cost cutting measures and considering moving to NEBNext Quick DNA Library prep. We have a 454 Junior and all of our kits and reagents are for rapid libraries. With NEBNext I have to order the adaptors separately. When I go to the IDT website, the only rapid library option for oligos includes the MIDs, which is fine. When I click on one of them, I tells me there is an Adaptor A and B. However, in the Roche rapid library protocol and the NEBNext protocol, when they refer to the adaptor ligation, it sounds like there is a single adaptor - not A and B. In the rapid library kits, there is a single tube of adaptor. The NEBNext protocol at the adaptor ligation step says to "add 1.0ul of adaptor to the reaction tube" with no mention of concentration. Am I supposed to mix MID adaptors we purchased from IDT in equimolar concentrations then add 1ul of that? At what concentration?
Thanks for any enlightenment.
Comment