HI,All:
I used trinity to assembly my rna data to get isoforms with the combined way.then maped my data to the sequence by the RSEM,but mapped read of one sample is 0.2% ;Trinity and RSEM ran successfully.Then I rebuided my sample library and resequenced the sample .Lucky the mapped read is more than 70%.
Now i meet the question again ,I donot get the reason.Anybody can help me ?Thanks
I used trinity to assembly my rna data to get isoforms with the combined way.then maped my data to the sequence by the RSEM,but mapped read of one sample is 0.2% ;Trinity and RSEM ran successfully.Then I rebuided my sample library and resequenced the sample .Lucky the mapped read is more than 70%.
Now i meet the question again ,I donot get the reason.Anybody can help me ?Thanks
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