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Old 07-12-2018, 11:25 AM   #1
Location: FL

Join Date: Sep 2012
Posts: 10
Question 16S sequencing from total RNA?

I'm working with a researcher that wants to identify the bacterial communities present in human dental pulp tissue, blood, and saliva from isolated RNA. What is the advantage (or disadvantage) from starting with RNA versus DNA? Typical 16S workflows use DNA as input with targeted 16S rRNA V3/V4 region amplification for library generation and long-read sequencing. This researcher has already performed DNA-Seq in a previous study but now "wants to confirm results through RNA"...
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16s rrna, metagenomics, nextseq

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