Hi,
I am trying to generate some statistics with picard CollectRnaSeqMetrics utility. The parameter STRAND_SPECIFICITY requires a value described as follows.
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For strand-specific library prep. For unpaired reads, use FIRST_READ_TRANSCRIPTION_STRAND if the reads are expected to be on the transcription strand. Required. Possible values: {NONE, FIRST_READ_TRANSCRIPTION_STRAND, SECOND_READ_TRANSCRIPTION_STRAND}
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my library is a paired library, so do I need to use value NONE? otherwise, can you tell me how to determine the correct value?
I am trying to generate some statistics with picard CollectRnaSeqMetrics utility. The parameter STRAND_SPECIFICITY requires a value described as follows.
----------------------------------
For strand-specific library prep. For unpaired reads, use FIRST_READ_TRANSCRIPTION_STRAND if the reads are expected to be on the transcription strand. Required. Possible values: {NONE, FIRST_READ_TRANSCRIPTION_STRAND, SECOND_READ_TRANSCRIPTION_STRAND}
---------------------------------
my library is a paired library, so do I need to use value NONE? otherwise, can you tell me how to determine the correct value?
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