Hi, our Genomics Center sent me a link to the MiSeq run they are doing for us with the new 600 bp chemistry - and I notice something on the run stats that scares me:
1. Yield perfect read 1: 170.8M
2. Yield perfect read 4 (second 300 bp read): 9.6M!
The run is still going- will the second read keep going up? It started at 8.9 M for this statistic, and I panicked because in the first attempt to sequence my amplicons, the second read was a lot lower than the first.
I'm worried because I added indexes and adapters via PCR (regular primer, then adapters+ regular primers, then indexes+adapters in 3 sequential PCRs) and I'm so afraid I screwed that up.
1. Yield perfect read 1: 170.8M
2. Yield perfect read 4 (second 300 bp read): 9.6M!
The run is still going- will the second read keep going up? It started at 8.9 M for this statistic, and I panicked because in the first attempt to sequence my amplicons, the second read was a lot lower than the first.
I'm worried because I added indexes and adapters via PCR (regular primer, then adapters+ regular primers, then indexes+adapters in 3 sequential PCRs) and I'm so afraid I screwed that up.
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