Hi,
have anyone tried to multiplex samples and targeted for higher coverage in samples vs. controls. Eg. as in the truseq exome enrichment one is expected to pool 500 ng of each library, but instead use 25 % control and 75% sample?
Another question: is it possible to go down in concentration say to 250 ng into the exome enrichment protocol, or is 500 ng an absolute lower limit?
Regards,
see
have anyone tried to multiplex samples and targeted for higher coverage in samples vs. controls. Eg. as in the truseq exome enrichment one is expected to pool 500 ng of each library, but instead use 25 % control and 75% sample?
Another question: is it possible to go down in concentration say to 250 ng into the exome enrichment protocol, or is 500 ng an absolute lower limit?
Regards,
see
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