DEXSeq results: an weird gene

[Jerry_Zhao]

Hi All,

I recently used DEXSeq to perform alternative splicing on one of my RNA-seq data.

After got the results, I noticed a weird gene.
From the plot attached, the few exons of this gene are clearly alternative spliced. However, DEXSeq assigned NA to the padj for these exons.

Anyone knows what happens?

Thanks!


Best,
Jerry

DEXSeq results for all exons on this gene:
groupID featureID exonBaseMean dispersion stat pvalue padj KO WT log2fold_WT_KO countData.KO1 countData.KO2 countData.WT1 countData.WT2

ENSMUSG00000024513:E001 ENSMUSG00000024513 E001 1 0.09 0.11 0.73 NA NA NA NA 1 0 2 1

ENSMUSG00000024513:E002 ENSMUSG00000024513 E002 72.75 0.001 147 7.15e-34 NA NA NA NA 74 77 90 50

ENSMUSG00000024513:E003 ENSMUSG00000024513 E003 179 0.0008 255 1.64e-57 NA NA NA NA 0 1 439 276

ENSMUSG00000024513:E004 ENSMUSG00000024513 E004 152.25 0.0009 226 2.7e-51 NA NA NA NA 0 0 373 236

ENSMUSG00000024513:E005 ENSMUSG00000024513 E005 17.5 0.006 183 8.87e-42 NA NA NA NA 37 31 0 2

ENSMUSG00000024513:E006 ENSMUSG00000024513 E006 225.5 0.001 35 2.12e-09 NA NA NA NA 42 51 486 323

ENSMUSG00000024513:E007 ENSMUSG00000024513 E007 316.25 0.004 5 0.02 NA NA NA NA 88 97 625 455

ENSMUSG00000024513:E008 ENSMUSG00000024513 E008 23.5 0.006 28 8.14e-08 NA NA NA NA 23 19 27 25

ENSMUSG00000024513:E009 ENSMUSG00000024513 E009 260.5 0.004 0.50 0.47 NA NA NA NA 106 95 480 361

ENSMUSG00000024513:E010 ENSMUSG00000024513 E010 8.25 0.011 16 4.8e-05 NA NA NA NA 9 8 9 7

ENSMUSG00000024513:E011 ENSMUSG00000024513 E011 2 0.05 0.25 0.61 NA NA NA NA 1 1 5 1

ENSMUSG00000024513:E012 ENSMUSG00000024513 E012 332.25 0.0005 9.50 0.002 NA NA NA NA 151 132 647 399

ENSMUSG00000024513:E013 ENSMUSG00000024513 E013 366.5 0.0013 7 0.006 NA NA NA NA 151 157 735 423

ENSMUSG00000024513:E014 ENSMUSG00000024513 E014 356 0.002 2 0.089 NA NA NA NA 158 127 729 410

ENSMUSG00000024513:E015 ENSMUSG00000024513 E015 469.75 0.001 0.9 0.34 NA NA NA NA 192 162 974 551