SEQanswers

Go Back   SEQanswers > General



Similar Threads
Thread Thread Starter Forum Replies Last Post
Newbie - Primer-Blast confusion brightelf Introductions 2 10-26-2015 07:00 AM
Miseq custom primer design, primer linker Cyrus Taheri Illumina/Solexa 1 04-21-2015 05:25 PM
Primer sequences emPCR and sequencing primer Ion Torrent laurakrol Sample Prep / Library Generation 1 04-25-2014 04:08 AM
problems with M13 universal tailed fusion PCR for 454 pyrosequencing melcar 454 Pyrosequencing 8 08-11-2013 02:44 PM
Titanium amplicon fusion primer confusion Phimes Sample Prep / Library Generation 3 01-17-2011 06:53 AM

Reply
 
Thread Tools
Old 02-25-2016, 11:33 PM   #1
nouba
Junior Member
 
Location: europe

Join Date: Mar 2014
Posts: 5
Default M13 primer confusion!

Hi!
I hope someone can help me with this new found interest, DNA sequencing!
I have heard about M13 primer, but I am not sure of what it is or how/when to use it?? Can someone please help me or guide me?
nouba is offline   Reply With Quote
Old 02-26-2016, 03:38 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,226
Default

M13 primer often is used to sequence or amplify DNA insert from vectors which have M13 primer sequence. This way one can sequence a cloned DNA fragment without knowing the insert sequences.
nucacidhunter is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 03:23 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO