SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
RNA-Seq: Comparative Analysis of RNA-Seq Alignment Algorithms and the RNA-Seq Unified Newsbot! Literature Watch 3 07-31-2011 07:08 PM

Reply
 
Thread Tools
Old 05-22-2019, 05:06 AM   #1
Micro151086
Junior Member
 
Location: Sheffield

Join Date: May 2019
Posts: 3
Default Would you go ahead with RNA-seq?

Hello,

I have 27 samples of total RNA isolated from E. coli which (having passed all initial requirements) have failed QC prior to sequencing. We are using a HiSeq 2 x 150 bp reads. The RINs are fine but the DV200 is low- the company have said this indicates degradation but looking at the tapestation trace it doesnt seem to be degraded. The large band of what I am presuming are tRNA is skewing the DV200. My main concern is considering we are mainly interested in the mRNA should we proceed with sequencing considering the large abundance of tRNA?
Attached Images
File Type: jpg RNA total.jpg (66.0 KB, 12 views)
Micro151086 is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 04:43 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO