SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
Bead removal of <1000 bp from LARGE fragments cement_head Sample Prep / Library Generation 4 04-04-2019 12:00 PM
DNA bead purification seqance General 6 06-01-2018 04:55 AM
Agencourt RNAclean XP bead purification troubleshooting Terazax Sample Prep / Library Generation 2 07-30-2015 07:00 AM
Ampure magnetic bead purification -- how does it work ? Christine90 Sample Prep / Library Generation 2 11-19-2012 04:16 AM
alternatives to sera-mag bead purification peromhc Sample Prep / Library Generation 1 05-12-2010 09:03 AM

Reply
 
Thread Tools
Old 08-18-2019, 09:30 PM   #1
Carcharodon
Member
 
Location: Honolulu, HI

Join Date: Jul 2015
Posts: 40
Default Removal of carry-over EtOH after bead purification of DNA

I recently purified some DNA (double-digest of genomic DNA) with SPRI beads, and I don't believe I let them dry long enough to get rid of excess EtOH used during the rinse step before eluting the DNA with HPLC water.

I read somewhere that placing the DNA solution on a SpeedVac for ~ 2 min should get rid of the ethanol, but I'm skeptical. Would that actually work, especially with such dilute EtOH?

Thanks in advance!
Carcharodon is offline   Reply With Quote
Old 08-19-2019, 11:05 AM   #2
teuthbrush
Junior Member
 
Location: California

Join Date: Aug 2018
Posts: 7
Default

For what it's worth, I just use a pipette, and even with a drying time of less than one minute, I haven't had any inhibition of my PCRs using Phusion as an enzyme, at least. Occasionally I'll see some weirdness on my Bioanalyzer trace, but it's hard to say whether that's the product of EtOH contamination.
teuthbrush is offline   Reply With Quote
Old 08-21-2019, 03:37 PM   #3
Carcharodon
Member
 
Location: Honolulu, HI

Join Date: Jul 2015
Posts: 40
Default

Quote:
Originally Posted by teuthbrush View Post
For what it's worth, I just use a pipette, and even with a drying time of less than one minute, I haven't had any inhibition of my PCRs using Phusion as an enzyme, at least. Occasionally I'll see some weirdness on my Bioanalyzer trace, but it's hard to say whether that's the product of EtOH contamination.
Thanks, that's reassuring.
Carcharodon is offline   Reply With Quote
Reply

Tags
ethanol, spri beads

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 10:14 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO