SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
nimblegen capture lid temperature weihuameng Sample Prep / Library Generation 0 07-08-2013 06:39 AM
5500 EZ Exome Hybridisation Enhancers JPC SOLiD 2 09-05-2011 01:02 AM
Baits length for exome capture? (Nimblegen and Aglient) sehrrot Sample Prep / Library Generation 3 04-04-2011 01:39 PM
Targeted sequence capture - Nimblegen + GS Junior sutree 454 Pyrosequencing 1 10-19-2010 05:53 AM
Nimblegen Seq Capture Vs RainDance Technologies ATGC_man 454 Pyrosequencing 0 11-20-2008 01:55 AM

Reply
 
Thread Tools
Old 10-30-2013, 03:05 AM   #1
kodbeeck
Junior Member
 
Location: Bel

Join Date: Jan 2013
Posts: 4
Default Nimblegen whole exome capture, hybridisation step

We are currently performing lib prep using nimblegen's SeqCap EZ whole exome capturing kit.

At the hybridisation step in the protocol, we have to incubate the samples at 47C for 64-72 hours.

For practical reasons, we can only continue the protocol after 4 days instead of the proposed 3.

We have several options:

1) hybridise the sample for 96 hours
2) hybridise samples for 72 hours and keep them at 4C untill we are ready to proceed
3) postpone the hybridisation step

As we want to sequence the samples ASAP, we prefer option 1 or 2.

Does anyone have experience with this? Please share.
kodbeeck is offline   Reply With Quote
Old 11-13-2014, 11:50 PM   #2
JeroenD
Junior Member
 
Location: Brussels

Join Date: May 2013
Posts: 3
Default

Hi,

Good question. I have the same issue. Which option did you eventually do? I'm thinking to do a hybridization of 92 hours. This shouldn't give any problems, right?
JeroenD is offline   Reply With Quote
Old 02-15-2015, 06:36 AM   #3
herter
Junior Member
 
Location: Saint Louis

Join Date: Feb 2015
Posts: 8
Default

Any problems with doing hybridization longer? 90 hours?
herter is offline   Reply With Quote
Old 04-28-2015, 03:00 AM   #4
drops
Junior Member
 
Location: Barcelona

Join Date: Nov 2011
Posts: 4
Default any expierience

...same here. Looking eagerly for an answer.
Thank you
drops is offline   Reply With Quote
Old 04-28-2015, 04:13 AM   #5
herter
Junior Member
 
Location: Saint Louis

Join Date: Feb 2015
Posts: 8
Default

Nimblegen rep confirmed that it can go longer, just make sure that nothing can evaporate.
herter is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 11:30 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO