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Old 01-30-2015, 07:45 AM   #1
Gregster
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Default zebrafish whole blood RNASeq libaray with globin

Is it possible to make a good whole blood RNASeq library without depleting globin?

I am just starting my adventure with RNASeq. I want to make an adult zebrafish whole blood RNASeq library, but I need total expression data and can not (would strongly prefer not to) exclude globin. Will it be challenging to get a good read from non-globin-depleted sample? Is it just a matter of increased number of reads? I found a paper dealing with embryonic zebrafish whole blood library where they didn't mention any globin depletion, but I'm not sure how it relates to an adult sample. Can I expect them to be similar in globin content? I have heard that globin content may differ between species, so I specified my model.
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Old 04-24-2019, 10:53 AM   #2
JTH-Genome
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Although not ideal, try the method described in this paper: https://www.nature.com/articles/srep31584
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