SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Costs zhiller RNA Sequencing 5 03-24-2016 05:25 PM
454 Roche Titanium Run Costs gendxdoc 454 Pyrosequencing 0 05-03-2010 04:41 PM
Happy Tiger Year! Xi Wang Introductions 0 02-12-2010 07:32 PM
Reagent costs per run gavin.oliver General 1 02-12-2010 03:40 AM
Complete installation costs for second-gen sequencer brjordan General 0 12-05-2009 07:21 AM

Reply
 
Thread Tools
Old 11-17-2011, 11:08 PM   #1
ca85
Junior Member
 
Location: Europe

Join Date: Oct 2011
Posts: 2
Default PE Sequencing or wait 1 year for costs do go down?

Hi,

we are planning our new sequencing experiment and are arguing about single end reads against paired end reads.

If we sequence 100bp PE reads instead of 100bp SE reads it would cost ~70% more. So our/my main question is:

Do you thing it's worth it to sequence PE reads, ie. is the gain in information worth the extra money?

As we have limited human resources, I think analyzing the data will last at least a year. Do you think by then it is cheaper to sequence it with PE reads again as the cost are going down, ie. could we get it done for the same prize = the 70% we are would now pay extra?


Any suggestions would be highly appreciated!!

All the best!
ca85 is offline   Reply With Quote
Old 12-09-2011, 01:11 AM   #2
Bukowski
Senior Member
 
Location: Aberdeen, Scotland

Join Date: Jan 2010
Posts: 355
Default

It would help to know what your experiment/application was in order to ascertain whether you would gain information with a PE approach..
Bukowski is offline   Reply With Quote
Old 12-09-2011, 05:39 AM   #3
Heisman
Senior Member
 
Location: St. Louis

Join Date: Dec 2010
Posts: 535
Default

If you really plan to wait a year you're almost guaranteed the price will drop at least close to 70% and possibly more. What type of project is this?
Heisman is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 03:37 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO