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Old 02-03-2020, 04:25 AM   #1
emlas
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Default 16S rRNA

I wonder where can I download a gtf file for microbial 16S rRNA (DNA)
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Old 02-03-2020, 05:20 AM   #2
GenoMax
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For analysis of 16S data you will want to use an established pipeline such as Qiime (link).
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Old 02-03-2020, 05:55 AM   #3
emlas
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Default 16s

Thank you very much, GenoMax! Is the any program supported by Windows?
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Old 02-03-2020, 06:55 AM   #4
emlas
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Default 16S rRNA

I am afraid that I might have caused a misunderstanding. I explain: I asked a question *I wonder where can I download a gtf file for microbial 16SrRNA (DNA) sequences*
I meant a gtf file of published microbial 16SrRNA (DNA) sequences. Not my own ones. I trust this aligned file of 16S rRNA does exist as the method has been around for such long time. I need it for Qlucore analysis.
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Old 02-03-2020, 04:23 PM   #5
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There are databases e.g. SILVA that provide this information. NCBI also has a 16S microbial dataset.

As for your question about a tool that can be used on windows, I don't know any that are free. There are commercial programs like CLC Genomics Workbench, Geneious that have windows versions to do NGS data analysis.
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