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Old 10-02-2011, 11:10 PM   #1
mwt
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Location: AU

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Question truseq libraries for nimblegen catpure

Hi
We've just started to used the truseq library prep for nimblegen exome capture. The NG capture protocol requires 1 ug of library but we can't seem to get more than 700-800 ng library?
Interested to know if anyone else has had the same experience.
MWT
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Old 11-18-2011, 08:08 AM   #2
Maqbool
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In some cases we did get up to 1 ug Library product but mostly not. It is very much dependent on the quality of the genomic DNA
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