Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Library prep for 50ng input with TruSeq DNA- or RNAseq adapters? sehrrot Sample Prep / Library Generation 1 11-09-2011 04:03 AM
QC of cDNA library prep for GA cybog337 Sample Prep / Library Generation 3 06-08-2011 10:10 AM
unexpected cDNA product during library prep amango Illumina/Solexa 4 08-11-2010 10:43 AM
RNA-Seq: cDNA prep and fragmentation questions ngcrawford Sample Prep / Library Generation 14 07-12-2009 05:57 AM
Question about Sequencing of mixed small RNA cDNA library satp Illumina/Solexa 2 06-19-2008 04:59 AM

Thread Tools
Old 06-05-2012, 05:30 PM   #1
Senior Member
Location: US

Join Date: Dec 2010
Posts: 431
Default How much cDNA out of RNA? - RNAseq library prep

When generating RNAseq libraries (using RNA fragmentation, the "standard" reverse transcription with , Superscript III, random hexamers, and second strand synthesis with DNA Polymerase I/RNase H) - how much DNA would one expect to generate from about 100 ng mRNA?

We have been getting about 50 ng DNA (on average 250 bp fragments, before adapter ligation) - is this amount in the expected range?

Thanks a lot in advance.
luc is offline   Reply With Quote

cdna, library prep, rnaseq

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 09:56 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO