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Old 06-18-2013, 12:42 AM   #1
GenomicIBK
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Location: Austria

Join Date: Nov 2011
Posts: 8
Lightbulb RNAseq (SOLiD) from 18 - 200 nt

We are interested in small non-coding RNAs. Whomever you ask about the size range of small RNAs, you get a different answer.
Lets assume, small RNAs range to 200 nt.

Is far as I know, there is the possibility of either small-RNA sequencing (miRNAs) OR 40 - 200 nt, but no combined protocol.

Has someone an idea, or is there a protocol/strategy out there which allows the sequencing of the 18 - 200 nt RNA fraction from total RNA with SOLiD? Or lets take 18 - 75 as this is the maximum readlength for SOLiD.
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non coding rna, protocol, rnaseq, small rna, solid 5500

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