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Old 01-19-2019, 11:18 PM   #1
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Location: Germany

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Default Crispr library which gene?

we're planning to do several Crispr runs to answer several question relating to translational machinery.

I was wondering whether there are prepared libraried for such experiments, or if one need to create his/her own library.
If I would like to answer different questions, does it make more sense to use several different libraries, or better to use one bigger, with more gene/sgRNA library.

The bigger question is how do I choose which genes should go into the library. Are there any parameters we should consider, or just take a genome-wide library and hope the gene we want are in it?
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