Another way to check if you have the reference genome. Map your reads to the reference genome, collect reads mapped to the forward strand and in coding regions, and count the number of reads starting at phase 1, 2 and 3 separately. If my hypothesis is correct, the 3 numbers will be statistically different and correlated with the GC% at the 3 phases.
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Originally posted by lh3 View PostAnother way to check if you have the reference genome. Map your reads to the reference genome, collect reads mapped to the forward strand and in coding regions, and count the number of reads starting at phase 1, 2 and 3 separately. If my hypothesis is correct, the 3 numbers will be statistically different and correlated with the GC% at the 3 phases.
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