I am about to prepare TruSeq PCR-free libraries for HiSeq X sequencing.
From:
"The supported insert size on the HiSeq X system is 350 bp and 450 bp for TruSeq Nano DNA and TruSeq DNA PCR-Free libraries."
As I see it this phrase could be interpreted in two ways; 350 bp for Nano and 450 bp for PCR-free, or both types of libraries can use either insert size. Anyone knows which it is? If the first; does that mean we MUST make our insert size 450 bp? There is not even a protocol for that in the TruSeq manual.
Jon
From:
"The supported insert size on the HiSeq X system is 350 bp and 450 bp for TruSeq Nano DNA and TruSeq DNA PCR-Free libraries."
As I see it this phrase could be interpreted in two ways; 350 bp for Nano and 450 bp for PCR-free, or both types of libraries can use either insert size. Anyone knows which it is? If the first; does that mean we MUST make our insert size 450 bp? There is not even a protocol for that in the TruSeq manual.
Jon
Comment