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Old 03-29-2011, 09:44 PM   #1
Br3ndan
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Location: Perth WA

Join Date: Mar 2011
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Default Rapid Library Adapter Dimer

Hi All,

We have recently done a 454 GS junior run of a rapid library build of some low-copy number aDNA and had an overwhelming number of reads appearing to be dimerisation of our y-adapters. Has anyone else experienced this?

Thanks
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Old 05-05-2011, 04:36 AM   #2
lalremruata
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Location: Germany

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What i did was made aDNA library using RAPID lib Kit. Amplified with emPCR primer and then cloned the amplified library to ensure fragment-adaptor ligation. of course my purpose was to enrich using NimbleGen array prior to 454 GS junior sequencing!!
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