SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Sanger/Dye Terminator



Similar Threads
Thread Thread Starter Forum Replies Last Post
Nextera XT AMPure XP product- no peak with Bioanalyzer? ADGM Illumina/Solexa 2 07-04-2015 06:18 AM
TruSeq DNA PCR-free Sample Preparation Kit (Part#15036187 Rev.A):extra peak, low qPCR snarapor Sample Prep / Library Generation 5 05-27-2014 05:03 AM
Alternating High/Low intensity of sample, but not PhiX BBthekid007 Illumina/Solexa 2 02-22-2012 06:36 AM
PCR product normalization Palecomic Sample Prep / Library Generation 0 01-20-2011 01:50 AM

Reply
 
Thread Tools
Old 12-15-2015, 09:43 AM   #1
sharones
Junior Member
 
Location: Chicago

Join Date: Dec 2015
Posts: 1
Default Seq of PCR product with great seq but one peak very low intensity

Hello,

I have not encountered anything like this before so I'm hoping someone could shed some light. I have a beautiful sequence for a PCR product and have noticed that although the rest of the sequence has uniform intensity, I have one peak that is so drastically diminished in its intensity that the software calls it a deletion, however, upon closer inspection the peak is very very small. What could cause this? Thanks!
sharones is offline   Reply With Quote
Old 12-16-2015, 05:58 AM   #2
MU Core
Member
 
Location: Columbia, Missouri

Join Date: Apr 2008
Posts: 52
Default

Sharones, would you be able to post the image of the peak trace?
MU Core is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 02:48 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO