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Old 12-28-2015, 10:23 PM   #1
mohd2b
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Default Rerun of cycle sequencing

Hi All

Recently I have run a cycle sequencing reaction, however the run was stopped due to a fatal error in the thermocycler. it stopped at cycle 8 of 25 cycles for approximately an hour till I realized the error message .then, I decided to rerun the cycle sequencing using different machine immediately.

from your experience , is the reaction is going to work if I sequence it. Should I stop and repeat the whole reaction again?

Thanks
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Old 12-29-2015, 07:25 AM   #2
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I assume you are using Big Dye v3 chemistry. I would expect the reaction to work as long as there were no other issues (i.e. primer is annealing, GC-rich template problems, etc.).
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Old 12-29-2015, 08:54 PM   #3
mohd2b
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Hi MU Core

iam using BigDye v1.1 chemistry.
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Old 12-30-2015, 06:50 AM   #4
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Hello mohd2b,

There should be no problem completing the cycles and loading the reaction. We've had a similar issue with a 96 well plate and data looked fine. Good luck.
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