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Old 11-03-2016, 01:40 PM   #1
Fragmented
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Location: BC

Join Date: Nov 2016
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Default 310 to 3130XL

Hello all,

Newbie to the forum here, and also new to the 3130XL! My lab is upgrading from the ABI 310 and I have a few stupid questions:

-can we continue to use the same POP4 polymer?

-do the septa actually need to be removed when denaturing the plate as the manual recommends? If so, what do you use in place of the septa?

-with the ABI 310, we use our own size standard and FAM/TET/HEX/TAM dyes. From what I read, I think we can set up our own 4 dye spectral calibration to set up a new matrix for each capillary. Is this correct?

-any other tips to save in time and money would be appreciated! As you all know, these machines (and their reagents) are not cheap!

Thanks in advance for your help!
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