Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Genome alignments

    Hello! My question is on how to make a "fake" reference genome from a set of contigs. I have the following data available:

    -Assembled contigs from my species (from genomic data, but I only have the contigs)
    -A reference genome (different species)
    -Linkage maps for both

    I would like to make an alignment between the contigs from my species and the reference, then concatenate the contigs from my species into a fake genome based on the order in the reference and/or linkage maps. The contigs are long (several kb), and I expect there to be many gaps due to introns, repeats, etc.

    Any suggestions for where to start? Thanks!

  • #2
    Take a look at Mummer for making the alignments.

    Comment


    • #3
      For mammalian genomes, bwa-sw.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Recent Advances in Sequencing Analysis Tools
        by seqadmin


        The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
        05-06-2024, 07:48 AM
      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin




        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
        04-22-2024, 07:01 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 05-10-2024, 06:35 AM
      0 responses
      19 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-09-2024, 02:46 PM
      0 responses
      22 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-07-2024, 06:57 AM
      0 responses
      21 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-06-2024, 07:17 AM
      0 responses
      21 views
      0 likes
      Last Post seqadmin  
      Working...
      X