Method to reuse Illumina flow cell

[cybog337]

Dear All,

I'm tyring to find any method to reuse illumina flow cell for SE.

If we can get the methods, I will reconstruct the new clusters after removing old cluster when I meet low number of clusters.

Do you have any good idea?

[ECO]

Moving to Illumina....Please next time try to hit the right forum with at least *one* of your posts...

[HESmith]

Sorry, but the flow cells are designed to be single-use. Cluster generation occurs by PCR with surface-tethered primers, followed by a cleavage reaction, to produce covalently-attached single-stranded sequencing templates.

The best strategy is to assess your library prior to clustering, by qPCR with adapter-specific primers.

-Harold

[lhemivw]

Do you have any idea？ the structure of flow cell

Do you have any idea？ the structure of flow cell

[james hadfield]

There is a paper from about 2005 or 2006 which describes how flowcells are made. You would need to do a lot of work to strip, recoat (silane or acrylamide I think) and graft the flowcell with the appropriate oligos. Almost certainly a waste of time and money as you would need to use a cluster station to do the work on as well.
If you get it to work well and can supply a method and cluster station protocol I suspect that would be a well read post!

[wangw]

Hi, James,

Can you please kindly give a detail infomation about the flowcell paper mentioned above, what journal, volume and title, etc? Thanks a lot.