Hello everyone,
I hope to find someone who can help. I want to use trimmomatic for single end Illumina reads from miRNA sequencing. I'm specifically unsure about the parameters: SLIDINGWINDOW and ILLUMINACLIP. I'm open to any suggestions, because I'm totally new to miRNA seq. RNA-seq I did before.
I really would appriciate your suggestions for this. Thanks
I hope to find someone who can help. I want to use trimmomatic for single end Illumina reads from miRNA sequencing. I'm specifically unsure about the parameters: SLIDINGWINDOW and ILLUMINACLIP. I'm open to any suggestions, because I'm totally new to miRNA seq. RNA-seq I did before.
Code:
-threads 1 -phred33 #encoding Sanger / Illumina 1.9 ILLUMINACLIP:./adapters/list.fa:2:30:10:6 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:17"
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