Hello,
I have RNA-seq data, which I am trying to analyze with DESeq. My file (.csv) appears to be correct
>head(myfile)
"
gene_id VZ_w13 VZ_w14a VZ_w14b VZ_w15a VZ_w15b VZ_w16a
1 ENSG00000253101 0 0 0 0 0 0
..."
However, when I try
>cds <- newCountDataSet(myfile,conds)
I get the following error message:
"Error in newCountDataSet(myfile, conds) : The countData is not integer."
The problem, as far as I can tell, is that my data are numerical, not integer, because when I run
>str(myfile)
"'data.frame': 53507 obs. of 14 variables:
$ VZ_w13 : num 0 0 0 0 8 0 0 0 0 0 ..."
Does anyone have a way to make my data integer, not numerical? As you can see, the data are in fact integers.
Thanks,
Stephan
I have RNA-seq data, which I am trying to analyze with DESeq. My file (.csv) appears to be correct
>head(myfile)
"
gene_id VZ_w13 VZ_w14a VZ_w14b VZ_w15a VZ_w15b VZ_w16a
1 ENSG00000253101 0 0 0 0 0 0
..."
However, when I try
>cds <- newCountDataSet(myfile,conds)
I get the following error message:
"Error in newCountDataSet(myfile, conds) : The countData is not integer."
The problem, as far as I can tell, is that my data are numerical, not integer, because when I run
>str(myfile)
"'data.frame': 53507 obs. of 14 variables:
$ VZ_w13 : num 0 0 0 0 8 0 0 0 0 0 ..."
Does anyone have a way to make my data integer, not numerical? As you can see, the data are in fact integers.
Thanks,
Stephan
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