Concentrating method for TruSeq lib prep in microRNA sequencing

[candicechu]

Hi all,

We are planning to submit our microRNA samples that we isolated from biofluids (serum and urine).

We use column-based isolation methods (e.g. mirVana PARIS) and we usually elude our samples in 50ul of RNase-free water; however, the requirement for sample submission (10-15 ng of microRNA in 5 ul) is way concentrated than our samples.

I was wondering how can we concentrate our microRNA samples? Does anybody have any experience with the vacuum method?

Any suggestions are appreciated. Thanks!

[nucacidhunter]

SpeedyVac volume reduction set at RT is a good method for your samples as they have been eluded with water and there is not any risk of salt accumulation or loss of sample.