Hi all,
It is totally new on the NGS world. Today, I started to prepare my first library preparation by using SureSelect Agilent technology. I did 6 samples (for now) and when I check the quantity/quality of my pre-capture by using the Bioanalyzer 1000 DNA chip I was not totally happy with the results. Effectively, all my 6 curves are more likely with the bad one (I attached an example). So, I understand that I have now several fragments around 300-700 pb. I was wondering about what could be happened? What I did wrong and if I can continue the protocol?
Thank you for your help and any tips will be well received regarding the library preparation
Regards,
David
It is totally new on the NGS world. Today, I started to prepare my first library preparation by using SureSelect Agilent technology. I did 6 samples (for now) and when I check the quantity/quality of my pre-capture by using the Bioanalyzer 1000 DNA chip I was not totally happy with the results. Effectively, all my 6 curves are more likely with the bad one (I attached an example). So, I understand that I have now several fragments around 300-700 pb. I was wondering about what could be happened? What I did wrong and if I can continue the protocol?
Thank you for your help and any tips will be well received regarding the library preparation
Regards,
David
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