Hello, I was trying to run a pcr for downstream illumina sequencing; and the primers are specifically designed for this purpose (with Illumina adapters and barcodes), so the primers end up been very long (>65 bp).
The regular primers (without Illumina adapters and barcodes) worked pretty well. However, the products from Illumina primers look like smears but not clear bands on the gel. Anyone have encountered the same problem before? Any ideas about the optimization would help. Thanks!
The regular primers (without Illumina adapters and barcodes) worked pretty well. However, the products from Illumina primers look like smears but not clear bands on the gel. Anyone have encountered the same problem before? Any ideas about the optimization would help. Thanks!
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