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Thread | Thread Starter | Forum | Replies | Last Post |
SRA to fastq conversion with fastq-dump loses sequences | pcantalupo | Bioinformatics | 13 | 10-08-2015 05:09 PM |
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fastq-dump for Illumina | duygu | Bioinformatics | 3 | 08-03-2011 03:00 PM |
Fastq-dump.exe | Giles | Bioinformatics | 2 | 06-11-2011 01:34 PM |
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#1 |
Junior Member
Location: Madison, WI Join Date: Sep 2015
Posts: 5
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I'm getting two errors on certain fastq-dump jobs. I am submitting jobs to HTCondor using a dag file to manage downloads. I am queuing multiple jobs and sometimes I get the following errors:
2016-03-31T03:17:24 fastq-dump.2.5.7 err: file unauthorized while opening file within network system module - log failure: RC( rcText,rcString,rcConverting,rcBuffer,rcInsufficient) in 'error with http open '$(U)'' 2016-03-31T03:17:24 fastq-dump.2.5.7 err: item not found while constructing within virtual database module - the path 'SRR0593 27' cannot be opened as database or table The strange thing is that sometimes I get the errors and sometimes I don't, even for the same files. For instance if I download 10 files, and 5 return these errors, if I submit the same ten jobs again, some or all of the files which gave errors might download normally. I'm very confused about this, any suggestions would be great. (I think the second error is the result of the first because these files seem to exist.) |
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#2 |
Simon Andrews
Location: Babraham Inst, Cambridge, UK Join Date: May 2009
Posts: 871
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If it's any consolation we gave up trying to understand all of the fastq-dump errors we saw and we now have a loop in our pipeline which simply tries the command again (up to 5 times) before deciding that there really is an error. a scary number of fastq-dump errors are not reproducible :-(
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Tags |
bioinformatics, sratoolkit fastq-dump |
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