Hello everyone
I am new to NGS. Currently, I am trying to prepare MethylC-seq libraries. How do I choose adapters for multiplexing? I read blog on multiplexing on Bioo Scientific but still have doubts. Perhaps someone here can help ...
1. How do I choose adapter index for multiplexing? - there should be equal balance of red and green but how do I choose adapter combination that will work-
Will combination of CTAGCT and GCGCTA work?
2. If base in position 1 in adapter 1 result in signal in red channel, does base in position 1 in adapter 2 need to result in green channel or can be red?
Look forward to your comments!
Cheers
I am new to NGS. Currently, I am trying to prepare MethylC-seq libraries. How do I choose adapters for multiplexing? I read blog on multiplexing on Bioo Scientific but still have doubts. Perhaps someone here can help ...
1. How do I choose adapter index for multiplexing? - there should be equal balance of red and green but how do I choose adapter combination that will work-
Will combination of CTAGCT and GCGCTA work?
2. If base in position 1 in adapter 1 result in signal in red channel, does base in position 1 in adapter 2 need to result in green channel or can be red?
Look forward to your comments!
Cheers
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