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Thread | Thread Starter | Forum | Replies | Last Post |
DeNovo assembly using pacBio data | krittika.sasmal | Pacific Biosciences | 50 | 06-05-2013 10:56 AM |
Denovo assembly | Thenna | Bioinformatics | 2 | 05-06-2013 07:09 AM |
parameters for Denovo assembly | nareshmvr | Illumina/Solexa | 10 | 03-01-2013 11:12 AM |
MIRA Question: Improving 454 assembly with RNAseq data | AaronS | De novo discovery | 0 | 01-13-2013 08:39 AM |
denovo assembly | nagaraj | Bioinformatics | 5 | 07-11-2012 07:13 AM |
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#1 |
Junior Member
Location: india Join Date: Nov 2011
Posts: 5
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Hi all,
I have RNAseq data of stress-control of various tissue of a plant. I am doing differential gene expression. In addition, cuffmerge provides me with transcripts assembled. But is this a valid transcriptome at publication ? Or should I go for a denovo assembly of the RNAseq data ? and for a comprehensive assembly of all the tissues together ? I have not come across publications reporting cuffmerge transcriptome. please advise. Thanks regards skm |
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Tags |
assembly, cuffmerge, rnaseq |
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