Hello,
I am dealing with problematic soil environmental samples with low HMW DNA and low yield. I have tried to make metagenomic libraries using Nextera XT kit but without luck.
What some other people have done in the field is to use whole genome amplification by multiple displacement amplification (MDA) to get enough DNA and then sequence it using 454.
What I was wondering is, could I use use Nextera library prep (input DNA 50ng) (not Nextera XT) instead of TruSeq library prep for Illumina on MDA amplified samples.
Has anyone seen/done/heard for MDA samples + Nextera (not in single cells).
While I am concerned with using only 50ng of input DNA when I get ug amounts from the MDA, we would be doing replication to capture more diversity of the sample.
Thank you.
I am dealing with problematic soil environmental samples with low HMW DNA and low yield. I have tried to make metagenomic libraries using Nextera XT kit but without luck.
What some other people have done in the field is to use whole genome amplification by multiple displacement amplification (MDA) to get enough DNA and then sequence it using 454.
What I was wondering is, could I use use Nextera library prep (input DNA 50ng) (not Nextera XT) instead of TruSeq library prep for Illumina on MDA amplified samples.
Has anyone seen/done/heard for MDA samples + Nextera (not in single cells).
While I am concerned with using only 50ng of input DNA when I get ug amounts from the MDA, we would be doing replication to capture more diversity of the sample.
Thank you.
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