Hi all,
I'm Soledad from Chile
I want to do amplicon sequencing, but I don't understand very well the neccesary kits.
My amplicons are 1400 bp length, so I think that I have to shear to make libraries. Do you know the ideal conditions of Bioruptor to obtain suitable fragments (150-250 bp) using 1400 bp DNA?
On the other hand, I have "ION DNA Barcode 1-16 kit" (Cat: 4468654) but I don't know which are the other kits that I need.
Also, searching in web, I found another kit: "ION Xpress Barcode Adapters 1-16 kit" (Cat: 4471250).
What's the difference? Which are the necessary kits to make a complete sequencing of amplicons?
HELP ME, PLEASE!!!!!
Thank you
Soledad
(sorry for my bad english)
I'm Soledad from Chile
I want to do amplicon sequencing, but I don't understand very well the neccesary kits.
My amplicons are 1400 bp length, so I think that I have to shear to make libraries. Do you know the ideal conditions of Bioruptor to obtain suitable fragments (150-250 bp) using 1400 bp DNA?
On the other hand, I have "ION DNA Barcode 1-16 kit" (Cat: 4468654) but I don't know which are the other kits that I need.
Also, searching in web, I found another kit: "ION Xpress Barcode Adapters 1-16 kit" (Cat: 4471250).
What's the difference? Which are the necessary kits to make a complete sequencing of amplicons?
HELP ME, PLEASE!!!!!
Thank you
Soledad
(sorry for my bad english)
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