Hello all,
I know RNA seq using SOLiD can be done from RNA isolated from a single cell which is in nanogram quanitity.
Now, we have a sample of DNA (circular plus linear) whose size ranges from 10 kb to 6 MB, isolated from some where. I would like to sequence it completely, like genomic DNA sequencing.
Question 1: How much DNA is needed to do it by either 454 sequencing or SOLiD sequencing?
Do we really need microgram or milligram quantities of DNA? If so, what is the use of advanced techs, for processing an archaeological sample?
Question 2: Can we do Exome sequencing of an unknown sample?
Any help is greatly appreciated.
Question 3: Any suggestions as best approach for complete sequencing or find out ORFs in the sample/
Venkata
I know RNA seq using SOLiD can be done from RNA isolated from a single cell which is in nanogram quanitity.
Now, we have a sample of DNA (circular plus linear) whose size ranges from 10 kb to 6 MB, isolated from some where. I would like to sequence it completely, like genomic DNA sequencing.
Question 1: How much DNA is needed to do it by either 454 sequencing or SOLiD sequencing?
Do we really need microgram or milligram quantities of DNA? If so, what is the use of advanced techs, for processing an archaeological sample?
Question 2: Can we do Exome sequencing of an unknown sample?
Any help is greatly appreciated.
Question 3: Any suggestions as best approach for complete sequencing or find out ORFs in the sample/
Venkata
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