Hi everyone,
I am still new to the next gen world. I have a question about read length. I am working with nextera custom enrichment, so our product is from 300 to 1000. I am still a little confused on how the cycling occurs. We are doing 150bp paired end sequencing, so what happens to the 1000bp sequences when it goes through 150 cycles? Is 700bps or so just not read? I hope this makes sense. I am just confused on how you perform say 150bp sequencing, but not exactly 150bp is in between the adapters.
Thanks in advance.
I am still new to the next gen world. I have a question about read length. I am working with nextera custom enrichment, so our product is from 300 to 1000. I am still a little confused on how the cycling occurs. We are doing 150bp paired end sequencing, so what happens to the 1000bp sequences when it goes through 150 cycles? Is 700bps or so just not read? I hope this makes sense. I am just confused on how you perform say 150bp sequencing, but not exactly 150bp is in between the adapters.
Thanks in advance.
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