Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Qiime parallel_pick_otus_uclust_ref.py

    Good morning everyone!

    I ran parallel_pick_otus_uclust_ref.py with a high quality fasta file and silva.nr.v119.align as the refence file.

    On the QIIME website it says you should get two files - a .txt and a .log file. However, I got a directory file 'POTU_gdTg_'. And when you open this directory you get several files - deletion_list.txt; expected_out_files.txt; merge_map.tt; POTU_gdTg_.0.fasta; POTU_gdTg_.1.fasta; POTU_gdTg_.2.fasta; POTU_gdTg_.3.fasta; POTU_gdTg_.4.fasta; POTU_gdTg_.5.fasta; POTU_gdTg_jobs.txt.

    Does anyone what these files mean? And which would I use to make the OTU tables? Is there another set before I can create the OTU tables? Do I need to assign taxonomy first?

    Thaaaaaanks!!!!

  • #2
    Are you sure this command is still valid?
    Usually, the Qiime OTU tables are in .biom file format since ~2 years.

    Comment


    • #3
      Thank you for your reply!

      Yes - the output of make_otu_table.py is a .biom file. But I don't have the tables - that is what I want to do now!

      I am wondering what file would be the input file for the make_otu_table.py command?

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Recent Advances in Sequencing Analysis Tools
        by seqadmin


        The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
        05-06-2024, 07:48 AM
      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin




        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
        04-22-2024, 07:01 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Today, 07:03 AM
      0 responses
      12 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-10-2024, 06:35 AM
      0 responses
      32 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-09-2024, 02:46 PM
      0 responses
      41 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-07-2024, 06:57 AM
      0 responses
      35 views
      0 likes
      Last Post seqadmin  
      Working...
      X