Hi guys,
I am generating RRBS library recently. In the whole process I use Beckman Ampure Xp beads to purify (cleanup and elute) DNA. But only the step of adapter ligation showed a extremely low efficiency of DNA yield (~35%).
The efficiencies of End repair and Add A steps are very good (over 90%). Do you have any experience about that? What should I do to improve the efficiency after adapter ligation?
Thank you so much!
I am generating RRBS library recently. In the whole process I use Beckman Ampure Xp beads to purify (cleanup and elute) DNA. But only the step of adapter ligation showed a extremely low efficiency of DNA yield (~35%).
The efficiencies of End repair and Add A steps are very good (over 90%). Do you have any experience about that? What should I do to improve the efficiency after adapter ligation?
Thank you so much!
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