Bubbles in Miseq Flowcell

DRYTCYV · 07-13-2014, 09:37 AM

Hi everyone,

I would like to know your opinion about the images in attachment.
The images are from different Runs, but this is the 5th Run that we detected strange things like this bubble.
I don’t know if you check the images in every Run, but for you, what kind of strange things you find in the images and assume that its normal?

Thanks

https://www.dropbox.com/s/32pva4lnde0zim4/Run%23C.png
https://www.dropbox.com/s/gvwxxr9rsg8yagc/Run%23B.png
https://www.dropbox.com/sc/er5vt25tb...e8-7hMm_EWELqa

dfhdfh · 07-14-2014, 12:03 AM

I observed similar things as well. These runs more or less failed with bad clustering efficiency. Illumina support is on the case but hasn't solved anything, yet (and I don't know whether the bubbles are the cause).

DRYTCYV · 07-15-2014, 08:15 AM

Hi dfhdfh,

Thank you for your feedback.
We alreday runned the Miseq test and there's no problem in the fluidic system, but our local support will be here tomorrow to change some part from the fluidic system.

No more feedback? anyone?

snetmcom · 07-18-2014, 06:07 PM

Bubbles are not that uncommon. It really depends on the frequency. I'd be curious if you find any tips.

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07-13-2014, 09:37 AM	#1
DRYTCYV Member Location: Portugal Join Date: Apr 2011 Posts: 50	Bubbles in Miseq Flowcell Hi everyone, I would like to know your opinion about the images in attachment. The images are from different Runs, but this is the 5th Run that we detected strange things like this bubble. I don’t know if you check the images in every Run, but for you, what kind of strange things you find in the images and assume that its normal? Thanks https://www.dropbox.com/s/32pva4lnde0zim4/Run%23C.png https://www.dropbox.com/s/gvwxxr9rsg8yagc/Run%23B.png https://www.dropbox.com/sc/er5vt25tb...e8-7hMm_EWELqa Last edited by DRYTCYV; 07-13-2014 at 09:47 AM.

07-14-2014, 12:03 AM	#2
dfhdfh Member Location: Germany Join Date: Jan 2013 Posts: 46	I observed similar things as well. These runs more or less failed with bad clustering efficiency. Illumina support is on the case but hasn't solved anything, yet (and I don't know whether the bubbles are the cause).

07-15-2014, 08:15 AM	#3
DRYTCYV Member Location: Portugal Join Date: Apr 2011 Posts: 50	Hi dfhdfh, Thank you for your feedback. We alreday runned the Miseq test and there's no problem in the fluidic system, but our local support will be here tomorrow to change some part from the fluidic system. No more feedback? anyone?

07-18-2014, 06:07 PM	#4
snetmcom Senior Member Location: USA Join Date: Oct 2008 Posts: 158	Bubbles are not that uncommon. It really depends on the frequency. I'd be curious if you find any tips.