Multi-Reads in ChIP-Seq Experiment

KyuzoSeq · 04-08-2015, 08:44 AM

I would like to ask about known literature about multiple reads of ChIP-Seq analysis.

Commonly researchers use "uniqely" mapped reads in their work. They blink reads which are aligned multiple locations. And we know that most alignment tools choose one of these multiple reads randomly.

This is an open problem of chip-seq analysis is waiting to solve.

There are few research using Expectation-Maximition algoritm to solve this. I would like to ask do you know any method to rescue multireads, any research or software? How scientifics handle or rescue with this multiple reads?

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04-08-2015, 08:44 AM	#1
KyuzoSeq Junior Member Location: Turkey Join Date: Mar 2015 Posts: 9	Multi-Reads in ChIP-Seq Experiment I would like to ask about known literature about multiple reads of ChIP-Seq analysis. Commonly researchers use "uniqely" mapped reads in their work. They blink reads which are aligned multiple locations. And we know that most alignment tools choose one of these multiple reads randomly. This is an open problem of chip-seq analysis is waiting to solve. There are few research using Expectation-Maximition algoritm to solve this. I would like to ask do you know any method to rescue multireads, any research or software? How scientifics handle or rescue with this multiple reads?