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Old 05-23-2011, 12:59 AM   #1
gatapishi
Junior Member
 
Location: Osaka, Japan

Join Date: Nov 2010
Posts: 3
Question Chip-seq or Medip-seq not for C57BL/6 but Balb/c

Hello all.
I am always enjoying this site and this is my first posting.

Now I am interested in epigenetics of lymphocytes.
For analyzing the influence of gene-deletion (knock out) on epigenetics
I would like to use genetically modified mice whose back ground is
balb/c not c57bl/g/6. But reference sequence information mm9 is
mainly made by sequencing C57BL/6j mice.

So I am worrying about the genetic difference between
balb/c and C57bl/6. Maybe important region is conserved, but there
are several regions not conserved between c57bl/6 and balb/c.

If there is anyone who have experience of analyzing balb/c with
chip-seq or medip-seq, Please advise me on this issue.

Thank you for your time.

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Old 09-06-2012, 11:54 PM   #2
Jones90
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Location: NSW

Join Date: Sep 2012
Posts: 2
Default A bit of a delay from the date of your post

Hi,

I'm just in reply to find out if you were able to work with MeDIP-seq using balb/c mice DNA?

If so do you know what the cost is per sample?

Thanks
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