Hi All,
We have some RNA samples from frozen tissue which we want to have sequenced using the Illumina platform. However, some of the samples have low RIN values on the Agilent Bioanalyzer. We would really like to get the sequence but can't afford to blow the money it would cost. We have a range of RIN values from 3.6 to 10. BGI (our potential sequence service provider) suggests a RIN > 7 for samples.
My question is, has anybody had a good (preferably!) experience of sequencing low RIN value RNA. Did you use DSN in the library prep?
Also, in another project, we are thinking of sending material from FFPE tissue - any experience there?
Thanks a lot,
Peadar
We have some RNA samples from frozen tissue which we want to have sequenced using the Illumina platform. However, some of the samples have low RIN values on the Agilent Bioanalyzer. We would really like to get the sequence but can't afford to blow the money it would cost. We have a range of RIN values from 3.6 to 10. BGI (our potential sequence service provider) suggests a RIN > 7 for samples.
My question is, has anybody had a good (preferably!) experience of sequencing low RIN value RNA. Did you use DSN in the library prep?
Also, in another project, we are thinking of sending material from FFPE tissue - any experience there?
Thanks a lot,
Peadar
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