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Old 08-10-2011, 07:22 AM   #1
whw
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Default DSN experts?

I'm looking for traces of microbial RNA in samples with large amounts of human RNA. Does anyone know if I can use DSN treatment to remove not only human rRNA/etc but also some of the less abundant human RNAs? The goal is to reduce the amount of human RNA to a level where (rare?) bacterial sequences can be detected and sequenced.

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Old 08-10-2011, 01:38 PM   #2
arolfe
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Not exactly what you were looking for, but this is a recently published, interesting approach to enriching for non-human RNA:

http://bioinformatics.oxfordjournals...7/15/2027.full
"Pathogen detection using short-RNA deep sequencing subtraction and assembly" by Isakov et al.
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Old 08-10-2011, 04:13 PM   #3
schandri
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I was wondering the same thing - whether you can monkey around with the the DNS Treatment or the incubation time before adding DNA so you remove lower abundant transcripts as well.
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Old 08-14-2011, 06:29 PM   #4
ScottC
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Yes. Illumina has some technical information about this. You should contact them to request it.
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